Native Mass Spectrometry Instrumentation

High resolution native Electrospray (ESI) Mass Spectrometry or Native MS has emerged as a standard tool in characterizing the structure and dynamics of biomolecular complexes. It can also provide insights in the stoichiometry and topology of subunits by inducing partial dissociation of the protein complex.

Since 2005 MS Vision has been active in the field of Native ESI Mass Spectrometry and has fulfilled a pioneering role in the development of the technique by developing dedicated high mass mass spectrometry systems. In close collaboration with the group of prof. Albert Heck at Utrecht University (Hecklab), we have gradually and steadily improved the technology for the analysis of intact protein complexes, often non-covalently bound, preserving native conditions in the gas phase.

As a small, dedicated and focused company, we have developed our technology through modification of Waters series Q-Tof and LCT instruments in response to specific user feedback. We moved on to translate what we had learnt to Synapt instruments, yielding excellent high mass performance and turning  Synapt instruments into versatile native MS platforms for structural biochemistry.

Why should you consider a high mass upgrade for native MS?

When working with intact proteins, the m/z values can increase because of a number of factors such as higher pH or loss of surface area upon complex formation. When using quadrupole based selection of ions, quadrupole mass filters have a certain maximum for selection of ions (typically assigned as the upper limit for selection. Commonly this is somewhere between 2.000 and 4.000 amu. However, transmission of ions goes down significantly above 3-4 times the upper limit for selection. Thus, for sensitive analysis of agglomerates, non-covalent complexes or native MS in general where m/z values of 6.000 or more are common, a high mass upgrade makes sense for optimal performance.

Mass spectrum of empty and loaded adeno-associated virus particles

What a High Mass Native Synapt systems provides to you:

  • Extension of the quadrupole m/z range to 32 kDa, enabling efficient transmission and selection of large protein complexes
  • Enhanced control over ion source and transfer region conditions, allowing for softer operation for better preservation of fragile proteins, and hotter conditions for enhanced activation of stable complexes
  • Enhanced activation and collisional cooling control in the TriWave region, allowing the admission of different gases into the IMS stage so it can either be used for post-activation cooling or Ion Mobility.
  • Customized acquisition settings, for best signal-to-noise in real-time monitoring
  • Preserving the Ion Mobility capability of the instrument

Applications range from the analysis of antibodies (Mw determination and purity) Antigen-Antibody interaction studies (stochiometry and affinity studies); Antibody PTM analysis (glycosylation, Antibody Drug Conjugates (ADC)); protein-protein and protein-ligand interactions (stochiometry, topology ) and virus capsids (composition, viral drugs development).

Native mass spectrometry is at the core of what we do! We develop and support these systems since the foundation of our company more than 15 years ago. By our own R&D we could build up a unique expertise in high mass and native mass spec and our specialists know the in’s and out’s of high mass MS. In the context of our R&D projects such as SuperMaMa we are continuously striving for further improvements in the field such as increased quadrupol mass ranges. So, expect even further expanded capabilities in the future!

Native mass spectrometry (native MS) literature using MS Vision instruments:


(When possible, the link provides direct access to the PDF file)

Your paper is not listed despite you used an MS Vision native MS system?

No problem! Send us a short message and we will add up to the list!

I would like to take the opportunity to thank you for the repair of our Quattro Micro and subsequent ‘hands on’ training course. The training course was very useful, generating positive feedbacks from the attendees. We look forward to receiving the training course certificates.

Dr. Nancy Weisbach
Across Barriers GmbH

Very happy with the professional survey of and repairs performed on our XEVO G2XS Q-ToF!

Anonymous from feedback form after Xevo QTOF G2-XS repair

Great Job done!

Anonymous from feedback form after Waters QTOF Ultima maintenance

The team of MS Vision has recently helped us to relocate our entire mass spectrometry lab, including five Orbitrap instruments (three Q Exactive series, one Elite and one Fusion) within the campus of Karolinska Institutet. Both the preparation and execution of this delicate operation was performed with absolute profession and accuracy. After the careful move, it took only two days to get all systems up and running like they would have never been down for a second. Needless to say how grateful we are for MS Vision for this excellent job!

Akos Vegvari, PhD
Proteomics Biomedicum, Karolinska Institutet

“I love how MS Vision makes us better!“

Boris Krichel

Excellent and very competent service as always. We are very satisfied. Thanks again that you made it possible at such short notice.

Anonymous from feedback form after Waters Synapt maintenance
University of Freiburg

The highly skilled service technicians of MS Vision are a keystone for us to achieve the highest uptime possible.

Dominic Helm

Very friendly and competent. We got a few new tips for our cleaning routine.

Anonymous from feedback form after Sciex API4000 maintenance
LMU Munich

Since last Wednesday we ran around 100 samples, everything is perfect (better sensitivity and linear dynamic range).  I have checked some data back to the last October, since I know what I am looking for, it seems that the charging problems started around December/January. Next time I will realize it much earlier.  Thank you both for your cleaning effort 😉


Manuel Mazenauer

Excellent service, as always. Many thanks Alex!

Anonymous from feedback form after LTQ-Orbitrap service

I would like to thank you again for your efforts in setting up our High Mass Q-Tof-MS and in introducing my students to the basic operating principles of the instrument. We performed the first measurements this week, which went really well. You did a great job and we are very happy with our new toy.

Prof. Dr. Andrea Sinz
Martin-Luther University Halle-Wittenberg

We are super happy that Jerre could fix our high mass QTOF II! It was a big task this time, but now I am confident we will be able to get good data with the instrument for many more years! The service is absolutely fantastic!!!

Anonymous from feedback form after High mass QTOF2 repair
Martin-Luther-Universität Halle-Wittenberg

MS Vision is our long lasting services partner for the maintenance and troubleshooting of our entire fleet of LC-MS/MS instruments. For us, their eagerness to extend their portfolio with new services and collaboratively identify ways of improvements is very much appreciated and fundamental in the reduction of instrument downtime that has been achieved.

Niels Martha
TLR International Laboratories

Perfect service!

Anonymous from feedback form after LTQ-Orbitrap XL relocation
Martin-Luther-Universität Halle-Wittenberg
MSVision Dedicated to Mass Spectrometry

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