The MS Vision High Mass Q-Tof opens the door to Mass Spectrometric studies of large, non-convalent protein complexes. It offers enhanced sensitivity for high mass species and allows tandem MS on precursor ions up to 30,000 m/z, turning proven Q-Tof technology into a unique instrument capable of producing groundbreaking results.

Standard Q-Tof instruments are limited in their capacity to analyze high mass species. This is due to many design characteristics which have been developed for optimal performance in proteomics applications. Performance for high mass applications can be improved by careful modification of individual instrumental details. MS Vision have bundled a large number of instrument modifications, developed over the past decade, into an incomparable analytical tool: the MS Vision High Mass Q-Tof.

Nearly every aspect of the instrument has been optimized for high mass work. Source pressure is increased, to allow better collisional cooling of the energetic, multiply charged ions. RF amplitudes in the transfer region are reduced to prevent RF breakdown. The quadrupole mass range is 30,000 and the maximum collisional energy has doubled to 400V. The collision cell operates at much higher pressures for better fragmentation and collisional cooling. Finally, the TOF mass range is no longer limited to 30,000.

In order to improve control over the crucial pressures in the source and collision cell, high quality gas- and vacuum handling equipment has been added along with localized pressure monitoring. The result of all this is an instrument which routinely gives you high mass performance you didn’t know could be achieved.

Talk from Christian Arlt, University of Halle, on native MS using an MS Vision modified QTOF II for protein complex characterization