Challenging the mass spectrometer
Overcoming the limitations of standard MS instrumentation in the analysis of large protein structures has been the focus of our efforts over the past decade. By working together closely with leading academic groups, we learnt how to get the best out of the mass spectrometer. The result is an instrument which allows researchers to unleash the full capacity of the Synapt in the analysis of intact proteins and complexes by native electrospray mass spectrometry.
Turning the Synapt into a Native Electrospray MS platform
The interest in MS of large biomolecules and biomolecular complexes has increased in recent years. Mass spectrometric study of intact proteins and their function in non-covalently bound complexes is providing more information about complicated biological systems. It is well known that intact large protein complexes can be transferred into the gas phase using nano-electrospray ionisation under native conditions, but subsequent MS or MS/MS analysis is prohibited by mass-scale, ion transmission or ion thermalisation limitations on standard instrumentation. These limitations have now been overcome, and all analytical tools available on the Synapt can be fully exploited in the study and analysis or large proteins and their complexes.
Making it work better
By careful consideration of the conditions that exist in the consecutive sections of the instrument, the performance of the Synapt for large ions can be improved considerably by changing the characteristics of the ion optical elements
All functionality of the original instrument is available on the Native Synapt. Simply choose between modes and analyze your samples. Soft conditions for preservation of complexes, high energy dissociation, Ion Mobility – it’s all possible!
Upgrade or full instrument
Whether you want to have your own Synapt upgraded or need a complete instrument, MS Vision has the solution. We offer full support by our highly skilled engineers so you can concentrate on running your samples.